发布于 由wu, bin

植物根系切片性状的测定

植物根系切片性状的测定

上接植物根系测定平台，这一次讲一讲我在测玉米根系性质的时候，如何观察根系切片性状

简介

Root Scan是一个java构建的对于根系横切面进行半自动图像分析的软件。其可以根系根系横切面图像的20多个性状，并自动划分气孔、皮层等结构。Root scan结果作为期刊发布于Plant and Soil. Volume 357, Numbers 1-2, 189-203 (2012)。具体下载方式请谷歌搜索宾夕法尼亚州立大学（penn state）Root Scan。
试例

操作过程

以下图为例进行，示例图片
在file目录下选择load image导入图片。点击start，程序自动识别图片轮廓

点击approve outline提取轮廓图片，点击process描绘全图轮廓

点击start phase two和approve outline显示标注中柱结构

在cortex下点击start three 和approve aerenchyma对皮层部分进行细胞识别和标注

最后点击finish，返回自动测定的横切面数据

测定项目

RXSA = Cross section area
TCA = Cortex area
TSA = Stele area
RatioCtoS = Cortex:Stele ratio
RatioCtoXS = Cortex:Cross Section ratio
RatioStoXS = Stele:Cross Section ratio
AA = Aerenchyma area
percCisA = Percentage of cortex that is aerenchyma
nonAA = Total cortex area - AA
MXVA = Metaxylem vessel area
percSisMX = Percentage of stele that is metaxylem
percXSisMX = Percentage of cross section that is metaxylem
MX_mean = Mean metaxylem size
MX_median = Median metaxylem size
MX_min = Minimum metaxylem size
MX_max = Maximum metaxylem size
MX_num = Number of metaxylem vessels
CF_num = Number of cell files
CCA = Cortex cell area
percCisCC = Percentage of cortex that is cortical cells
percXSisCC = Percentage of cross section that is cortical cells
CC_mean = Mean cortical cell size
CC_median = Median cortical cell size
CC_num = Number of cortical cells
Z0andEpi_mean = Mean of all cells in zone zero and the epidermis
Z0_mean = Mean of all cells in zone zero (edge)
Z1_mean = Mean of all cells in zone one (middle)
Z2_mean = Mean of all cells in zone two (center)
Z0andEpi_median = Median of all cells in zone zero and the epidermis
Z0_median = Median of all cells in zone zero (edge)
Z1_median = Median of all cells in zone one (middle)
Z2_median = Median of all cells in zone two (center)
Z0andEpi_num = Number of all cells in zone zero and the epidermis
Z0_num = Number of all cells in zone zero (edge)
Z1_num = Number of all cells in zone one (middle)
Z2_num = Number of all cells in zone two (center)
LA_mean = Mean lacunae size
LA_median = Median lacunae size
LA_min = Minimum lacunae size
LA_max = Maximum lacunae size
LA_num = Number of lacunae
Files start counting from 0 at the epidermis
Ratings indicate image quality, 0 the worst, 10 the best

实验室使用

在实验室中对植物根系横切面进行观察，可以配置一解剖镜，一显微镜，显微镜上附转化头将图片直接传输到计算机的rootscan上来实现快速、大批量的植物根系横切面观察。通过与之前的DIRT平台，可以快速对大批量的植物根系性状进行测定。